ENPP-1 Antibody [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
FITC

ENPP-1 Antibody [FITC] Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human ENPP1 protein (between residues 900-925) [UniProt P22413]
Localization
Membrane.
Clonality
Polyclonal
Host
Goat
Gene
ENPP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Peptide ELISA
Application Notes
This ENPP1 antibody is useful for Peptide ELISA, Western Blot and Immunohistochemistry-paraffin embedded sections. In Western blot a band was observed at ~130-150kDa in Human Liver and HepG2 lysates that was blocked by preincubation with the immunizing peptide. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Immunogen sequence has 85% homology to mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ENPP-1 Antibody [FITC]

  • ectonucleotide pyrophosphatase/phosphodiesterase 1
  • E-NPP 1
  • ENPP1
  • ENPP-1
  • Ly-41 Antigen
  • M6S1
  • M6S1NPP1
  • Membrane component chromosome 6 surface marker 1
  • membrane component, chromosome 6, surface marker 1
  • NPP1
  • NPPS
  • NPPSalkaline phosphodiesterase 1
  • PC1
  • PC-1
  • PCA1
  • PCA1ARHR2
  • PDNP1
  • PDNP1ectonucleotide pyrophosphatase/phosphodiesterase family member 1
  • Phosphodiesterase I/nucleotide pyrophosphatase 1
  • plasma-cell membrane glycoprotein 1
  • Plasma-cell membrane glycoprotein PC-1

Background

ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase family member 1) is a type II transmembrane glycoprotein which is implicated in membrane surface ATP hydrolysis, regulation of pyrophosphate levels, bone mineralization as well as soft tissue calcification. ENPP1 negatively regulates bone mineralization by hydrolyzing extracellular nucleotide triphosphates (NTPs) to produce inorganic pyrophosphate (PPi, an inhibitor of mineralization), whereas tissue-nonspecific ALP positively regulates mineralization by hydrolyzing NTPs and PPi to produce extracellular concentrations of inorganic phosphate (Pi, a substrate for mineralization). ENPP1 is expressed on outer surfaces of mineralizing cells, such as osteoblasts and chondrocytes, and on the membranes of osteoblast- and chondrocyte-derived matrix vesicles. It also hydrolyzes other NTPs such as GTP, CTP, TTP, UTP etc. to their corresponding monophosphates with the release of PPi and diadenosine polyphosphates, and also 3',5'-cAMP to AMP, and may also regulate the availability of nucleotide sugars in ER/Golgi complex, as well as the regulation of purinergic signaling. ENPP1 binds directly to INSR for inhibiting insulin signaling and ENPP1's K173Q/ K121Q polymorphism is associated with insulin resistance, NIDDM, and obesity. Defective ENPP1 causes increased susceptibility for ossification of the posterior longitudinal ligament of spine (OPLL), arterial calcification of infancy, generalized, type 1 (GACI1), obesity/glucose intolerance/NIDDM, and rickets hypophosphatemic autosomal recessive type 2 (ARHR2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ENPP1
Entrez