ENPP-1 Overexpression Lysate (Native)

Images

 
Western Blot: ENPP1 Overexpression Lysate (Adult Normal) [NBL1-10273] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ENPP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ENPP-1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209222 and based on accession number NM_006208. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), mRNA.
Gene
ENPP1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
99.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ENPP-1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ENPP-1 Overexpression Lysate (Native)

  • ectonucleotide pyrophosphatase/phosphodiesterase 1
  • E-NPP 1
  • ENPP1
  • ENPP-1
  • Ly-41 Antigen
  • M6S1
  • M6S1NPP1
  • Membrane component chromosome 6 surface marker 1
  • membrane component, chromosome 6, surface marker 1
  • NPP1
  • NPPS
  • NPPSalkaline phosphodiesterase 1
  • PC1
  • PC-1
  • PCA1
  • PCA1ARHR2
  • PDNP1
  • PDNP1ectonucleotide pyrophosphatase/phosphodiesterase family member 1
  • Phosphodiesterase I/nucleotide pyrophosphatase 1
  • plasma-cell membrane glycoprotein 1
  • Plasma-cell membrane glycoprotein PC-1

Background

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq]. Sequence Note: The translation start codon selected for this RefSeq is conserved in several species. A possible downstream start codon would reduce the protein length by 52 aa, and this start codon is referred to in PMID:2211644. No experimental evidence exists regarding which start codon is used. This RefSeq record was created from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to extend the N-terminus based on the presence of an upstream translation start codon that is conserved in several species. A possible downstream start codon would reduce the protein length by 52 aa. The downstream start codon is referred to in PMID:2211644. No experimental evidence exists regarding which start codon is used. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ENPP-1 Lysate (NBL1-10273) (0)

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Bioinformatics

Gene Symbol ENPP1
Entrez