Endophilin A1/SH3GL2 Antibody [Alexa Fluor® 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

Endophilin A1/SH3GL2 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Endophilin A1/SH3GL2
  Ala217-Pro293
  Accession # Q99962
• Specificity: Detects human, mouse, and rat Endophilin A1/SH3GL2 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endophilin A1/SH3GL2 Antibody [Alexa Fluor® 594]

• CNSA2
• CNSA2FLJ25015
• EEN-B1
• EEN-B1bA335L15.1 (SH3-domain GRB2-like 2)
• Endophilin A1
• Endophilin-1
• endophilin-A1
• FLJ20276
• SH3 domain protein 2A
• SH3 domain-containing GRB2-like protein 2
• SH3D2A
• SH3D2AEndophilin A1 BAR domain
• SH3-domain GRB2-like 2
• SH3GL2
• SH3P4
• SH3P4endophilin-1

Background

Endophilin A1 (also SH3GL2, SH3P4, EEN-B1, CNS-A2, GL2 and SH3D2A) is a 39-42 kDa member of the A subfamily, endophilin (attracted to endocytic protein) family of molecules. It is a neuronally-expressed cytosolic protein that is concentrated in presynaptic areas. Endophilin A1 is a key player in clathrin-associated endocytosis, participating in invagination plus vesicle fission and recycling. It is able to impact membrane curvature through electroststic differences via its N‑terminus, and interact with multiple synaptic region proteins such as dynamin and synaptojanin through an SH3 homology domain at its C-terminus. Although Endophilin A1 is a monomer in the cytosol, it will homodimerize and heterodimerize with endophilin-2 when active at the synapse. Endophilin A1 is also associated with endocytic trafficking of growth factor receptors. In concert with CIN85, Cbl and ataxin-2, Endophilin A1 extend the half-life of EGFR at the plasma membrane. Human Endophilin A1 is 352 amino acids (aa) in length. It contains an aforementioned N-terminal BAR domain (aa 18-249) plus an SH3 domain that binds Pro‑rich aa sequences (aa 290-349). There is one potential alternative start site at Met36. Endophilin A1 is one of three groupA endophilin isoforms, all of which are the product of separate genes. Although the -A2 and -A3 isoforms share approximately 70% aa overall sequence identity with -A1, over aa 217-293, -A2 and -A3 share less than 50% aa sequence identity with Endophilin A1. Over this same aa sequence, human and mouse Endophilin A1 share more that 97% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.