Emerin Antibody (3B9)

Western Blot: Emerin Antibody (3B9) [H00002010-M01] - EMD monoclonal antibody (M01), clone 3B9 Analysis of EMD expression in HeLa.

Immunocytochemistry/Immunofluorescence: Emerin Antibody (3B9) [H00002010-M01] - Analysis of monoclonal antibody to EMD on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Emerin Antibody (3B9) [H00002010-M01] - Analysis of monoclonal antibody to EMD on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.

Sandwich ELISA: Emerin Antibody (3B9) [H00002010-M01] - Detection limit for recombinant GST tagged EMD is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

Emerin Antibody (3B9) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EMD (AAH00738, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRLSPPSSSAASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPES
• Specificity: EMD - emerin (Emery-Dreifuss muscular dystrophy)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EMD
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Emerin Antibody (3B9)

• emerin
• Emery-Dreifuss muscular dystrophy
• LEM domain containing 5
• STAEDMDLEMD5

Background

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EMD
• Entrez:
  • 2010(Human)
• OMIM:
  • 300(Human)
• Uniprot:
  • AAH00738(Human)