ELP2 Antibody

Western Blot: ELP2 Antibody [NBP2-16321] - Sample (20 ug ) A: HeLa nucleus 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ELP2 Antibody [NBP2-16321] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ELP2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: ELP2 Antibody [NBP2-16321] - Analysis of paraffin-embedded human colon carcinoma, using ELP2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation: ELP2 Antibody [NBP2-16321] - HeLa whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ELP2 protein by 2 ug of ELP2 antibody 7.5% SDS-PAGE The immunoprecipitated ELP2 protein was detected by ELP2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Product Discontinued

ELP2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ELP2. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for ELP2 Antibody

• elongation protein 2 homolog (S. cerevisiae)
• elongator complex protein 2
• elongator protein 2
• FLJ10879
• SHINC-2
• signal transducer and activator of transcription 3 interacting protein 1
• signal transducer and activator of transcription interacting protein 1
• STAT3-interacting protein 1
• STATIP1
• StIP
• StIP1

Background

Regulates the ligand-dependent activation of STAT3. Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ELP2 Antibody (NBP2-16321)(2)

Publications using NBP2-16321

• Xia C, Ou S, Yang Y et al. ELP2-NLRP3-GSDMD/GSDME-mediated pyroptosis is induced by TNF-? in MC3T3-E1 cells during osteogenic differentiation Journal of cellular and molecular medicine 2023-10-13 [PMID: 37830762] (WB, IP, Mouse)
• Xu C, Xia C, Ou S et al. ELP2 -NLRP3-GSDMD/GSDME mediated pyroptosis induced by TNF- alpha during osteogenic differentiation Research Square 2022-08-19 (WB, Human)

Bioinformatics

• Gene Symbol: ELP2
• Uniprot:
  • Q6IA86(Human)