ELMSAN1 Antibody - BSA Free

Western Blot: ELMSAN1 Antibody [NBP1-71912] - analysis of ELMSAN1 in U87 whole cell lysates using anti-ELMSAN1 antibody. Lane 1 - contorl siRNA, Lanes 2 and 3 - U87 cells transfected with siRNAs targeting ELMSAN1. Image from verified customer review.

Western Blot: ELMSAN1 Antibody [NBP1-71912] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-C14orf43 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C14orf43 was also immunoprecipitated by another rabbit anti-C14orf43 antibody, which recognizes an upstream epitope.

• Reactivity: Hu
• Applications: WB, IP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ELMSAN1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ELMSAN1 Antibody - BSA Free (NBP1-71912) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 550 and 600 of human ELMSAN1 Open Reading Frame 43 using the numbering given in entry NP_001036783.1 (GeneID 91748).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELMSAN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ELMSAN1 Antibody - BSA Free

• c14_5541
• C14orf117
• C14orf43
• chromosome 14 open reading frame 117
• chromosome 14 open reading frame 43
• ELM2 and Myb/SANT-like domain containing 1
• ELMSAN1
• FLJ00335
• hypothetical protein LOC91748
• LSR68

Background

Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene. The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo. Chimera RNAi has many advantages over the conventional siRNAs. First, it has been demonstrated to have reliable knock-down for over 10,000 human genes. Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence. Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected. Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for ELMSAN1 Antibody (NBP1-71912) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 06/24/2015

Summary

• Application: Western Blot
• Sample Tested: U87 whole cell lysates
• Species: Human

Bioinformatics

• Gene Symbol: ELMSAN1
• Uniprot:
  • NP_001036783.1(Human)