Elk-1 [p Ser383] Antibody

Product Discontinued

Elk-1 [p Ser383] Antibody Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Elk-1, around the phosphorylation site of serine 383 (T-L-SP-P-I).
• Modification: p Ser383
• Specificity: Elk-1 (specific to Phospho-Ser383) detects endogenous levels of Elk-1 only when phosphorylated at serine 383.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Elk-1 [p Ser383] Antibody

• Elk1
• Elk-1
• ELK1, member of ETS oncogene family
• ETS domain-containing protein Elk-1
• ETS-like gene 1

Background

The transcription factor Elk1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. The serum reponse element in the promoter of the c-fos proto-oncogene. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk1. Elk1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 (Ser383) is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus. Elk-1 is a member of the Ets family of transcription factors (TCF) subfamily. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELK1
• Entrez:
  • 2002(Human)
  • 13712(Mouse)
  • 314436(Rat)
• Uniprot:
  • P19419(Human)