Elk-1 [p Ser383] Antibody

Images

 
Western Blot: Elk-1 [p Ser383] Antibody [NB100-81904] - Analysis of extract from HeLa cells untreated or treated with UV using Elk-1 (phospho-Ser383) antibody NB100-81904.
Immunohistochemistry-Paraffin: Elk-1 [p Ser383] Antibody [NB100-81904] - Analysis of paraffin-embedded human breast carcinoma tissue using Elk-1 (phospho-Ser383) antibody NB100-81904. The image on the right shows ...read more
Western Blot: Elk-1 [p Ser383] Antibody [NB100-81904] - Analysis of extracts from HT29 cells untreated or treated with UV using ELK1 [p Ser383] antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-81904
    • Availability
      Product Discontinued

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Elk-1 [p Ser383] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Elk-1, around the phosphorylation site of serine 383 (T-L-SP-P-I).
Modification
p Ser383
Specificity
Elk-1 (specific to Phospho-Ser383) detects endogenous levels of Elk-1 only when phosphorylated at serine 383.
Clonality
Polyclonal
Host
Rabbit
Gene
ELK1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Elk-1 [p Ser383] Antibody

  • Elk1
  • Elk-1
  • ELK1, member of ETS oncogene family
  • ETS domain-containing protein Elk-1
  • ETS-like gene 1

Background

The transcription factor Elk1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. The serum reponse element in the promoter of the c-fos proto-oncogene. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk1. Elk1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 (Ser383) is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus. Elk-1 is a member of the Ets family of transcription factors (TCF) subfamily. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ELK1
Entrez
Uniprot