Elk-1 Antibody (SU32-05)

Western Blot: Elk-1 Antibody (SU32-05) [NBP2-67459] - Analysis of ELK1 on human lung lysates using anti-ELK1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Elk-1 Antibody (SU32-05) [NBP2-67459] - Staining ELK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Elk-1 Antibody (SU32-05) [NBP2-67459] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Elk-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Elk-1 Antibody (SU32-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Elk-1 aa 378-428 / 428. (SwissProt: P19419 Human; SwissProt: P41969 Mouse; SwissProt: A4GTP4 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ELK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Elk-1 Antibody (SU32-05)

• Elk1
• Elk-1
• ELK1, member of ETS oncogene family
• ETS domain-containing protein Elk-1
• ETS-like gene 1

Background

ELK is a member of the family of genes homologous to the v-Ets oncogene isolated from the E26 eythroblastosis virus. Members of this family exhibit varied patterns of tissue expression. They also share a highly conserved C-terminal domain that may be responsible for the DNA binding activity of all members of the Ets gene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELK1