ELF3/ESE-1 Overexpression Lysate (Native)

Images

 
Western Blot: ESE1 Overexpression Lysate (Adult Normal) [NBL1-10230] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ESE1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ELF3/ESE-1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200631 and based on accession number NM_004433. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens E74-like factor 3 (ets domain transcription factor, epithelial-specific ) (ELF3), transcript variant 1, mRNA.
Gene
ELF3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ELF3/ESE-1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ELF3/ESE-1 Overexpression Lysate (Native)

  • E74-like factor 3 (ets domain transcription factor, epithelial-specific )
  • E74-like factor 3
  • ELF3
  • Epithelial-restricted with serine box
  • epithelial-specific)
  • Epithelium-restricted Ets protein ESX
  • Epithelium-specific Ets transcription factor 1
  • EPR1
  • EPR-1
  • ERT
  • ESX
  • ets domain transcription factor, serine box (epithelial-specific)
  • ETS-related transcription factor Elf-3
  • JEN
  • serine box

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF6146
Species: Hu
Applications: ICC
H00002629-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP1-69295
Species: Hu
Applications: WB, ICC/IF, IHC
NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-22169
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-33320
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NB120-7164
Species: Hu, Mu, Rt, Fi
Applications: ELISA, ICC/IF, IHC-Fr, MiAr
NB300-164
Species: Hu, Po, Ca, Rt(-)
Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP

Publications for ELF3/ESE-1 Lysate (NBL1-10230) (0)

There are no publications for ELF3/ESE-1 Lysate (NBL1-10230).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ELF3/ESE-1 Lysate (NBL1-10230) (0)

There are no reviews for ELF3/ESE-1 Lysate (NBL1-10230). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ELF3/ESE-1 Lysate (NBL1-10230) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ELF3/ESE-1 Products

Bioinformatics Tool for ELF3/ESE-1 Lysate (NBL1-10230)

Discover related pathways, diseases and genes to ELF3/ESE-1 Lysate (NBL1-10230). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ELF3/ESE-1 Lysate (NBL1-10230)

Discover more about diseases related to ELF3/ESE-1 Lysate (NBL1-10230).
 

Pathways for ELF3/ESE-1 Lysate (NBL1-10230)

View related products by pathway.

PTMs for ELF3/ESE-1 Lysate (NBL1-10230)

Learn more about PTMs related to ELF3/ESE-1 Lysate (NBL1-10230).
 

Research Areas for ELF3/ESE-1 Lysate (NBL1-10230)

Find related products by research area.

Blogs on ELF3/ESE-1

There are no specific blogs for ELF3/ESE-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ELF3/ESE-1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol ELF3
Entrez