ELF3/ESE-1 Antibody (662533) [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 662533
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

ELF3/ESE-1 Antibody (662533) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human ELF3
  Met1-Gly173
  Accession # P78545
• Specificity: Detects human and mouse ELF3 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ELF3/ESE-1 Antibody (662533) [Alexa Fluor® 750]

• E74-like factor 3 (ets domain transcription factor, epithelial-specific )
• E74-like factor 3
• ELF3
• Epithelial-restricted with serine box
• epithelial-specific)
• Epithelium-restricted Ets protein ESX
• Epithelium-specific Ets transcription factor 1
• EPR1
• EPR-1
• ERT
• ESX
• ets domain transcription factor, serine box (epithelial-specific)
• ETS-related transcription factor Elf-3
• JEN
• serine box

Background

ELF3 (E74-Like Factor 3), also known as ESE-1, ESX, ERT and JEN, is a 41-43 kDa member of the ETS family of proteins.  During uncomplicated (non-inflammatory) periods of cell differentiation, ELF3 is expressed exclusively by epithelial cells, repressing genes needed during early differentiation, and promoting genes needed for full differentiation.  Under conditions of inflammation, cells such as monocytes, endothelial cells and chrondrocytes express ELF3 and produce molecules such as Ang1 and COX2.  Human ELF3 is 371 amino acids (aa) in length.  It contains one PNT/pointed dimerization domain (aa 46-132), a protein stabilizing PEST sequence (aa 210-225), an A/T Hook region that binds to AT-rich DNA sequences (aa 236-252), and an ETS DNA binding domain (aa 273-355).  ELF3 interacts with CREBBP, EP300, KU70 and KU86.  There is one splice variant that shows a deletion of aa 174-200.  Over aa 1-173, human ELF3 shares 87% aa identity with mouse ELF3.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.