ELA2A Overexpression Lysate (Native)

Images

 
Western Blot: ELA2A Overexpression Lysate (Adult Normal) [NBL1-10218] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ELA2A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ELA2A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202746 and based on accession number NM_033440. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens elastase 2A (ELA2A), mRNA.
Gene
CELA2A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ELA2A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ELA2A Overexpression Lysate (Native)

  • chymotrypsin-like elastase family member 2A
  • chymotrypsin-like elastase family, member 2A
  • EC 3.4.21
  • EC 3.4.21.71
  • ELA2Apancreatic elastase 2
  • elastase 2A
  • elastase-2A
  • pancreatic elastase IIA
  • PE-1

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CELA2A