eIF5A2 Antibody

Western Blot: eIF5A2 Antibody [NBP2-16312] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 15% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: eIF5A2 Antibody [NBP2-16312] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EIF5A2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP2-16312] - Immunohistochemical analysis of paraffin-embedded Gastric carcinoma, using antibody at 1:500 dilution.

Product Discontinued

eIF5A2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human EIF5A2. The exact sequence is proprietary.
• Localization: Cytoplasm (By similarity); Nucleus (By similarity); Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Nucleus; nuclear pore complex (By similarity)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF5A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for eIF5A2 Antibody

• EIF-5A2
• eIF-5A-2
• eIF5AII
• Eukaryotic initiation factor 5A isoform 2
• eukaryotic initiation factor 5A
• eukaryotic translation initiation factor 5A2
• eukaryotic translation initiation factor 5A-2

Background

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EIF5A2
• Uniprot:
  • Q9GZV4(Human)