eIF5A2 Antibody

Western Blot: eIF5A2 Antibody [NB200-133] - Detection of endogenous and FLAG-tagged eIF5A2 in HeLa cells using NB 200-133 at a 1:1000 dilution

Product Discontinued

eIF5A2 Antibody Summary

• Immunogen: A synthetic peptide representing the C-terminus of the Human EIF5 A2 protein.
• Specificity: Reacts with the human EIF5 A2 protein. Cross-reactivity with human eIF5 A1 protein has been shown in endogenous samples.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF5A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: Western Blotting

Reactivity Notes

Human. Others not unknown.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF5A2 Antibody

• EIF-5A2
• eIF-5A-2
• eIF5AII
• Eukaryotic initiation factor 5A isoform 2
• eukaryotic initiation factor 5A
• eukaryotic translation initiation factor 5A2
• eukaryotic translation initiation factor 5A-2

Background

Eukaryotic translation initiation factor 5A2 (eIF5A-2), a recently discovered oncogene member of the eIF5A family, is an mRNA-binding protein implicated in the translation elongation process and it exerts a critical role at the level of mRNA turnover, probably acting downstream of decapping. eIF5A-2 involves in actin dynamics, progression of cell cycle, mRNA decay, stress response signaling as well as in the preservation of cellular wall's integrity. eIF5A-2 mediates the effects of polyamines on neuronal process extension/survival, and has been suggested to play a role in CNS development/functions as well as differentiation of skeletal muscle stem cells. eIF5A-2 has been reported to localize in nucleus, cytoplasm and ER membranes of cells, and hypusine modification, a posttranslational modification of at least one lysine residue to form hypusine [N-epsilon-(4-aminobutyl)lysine], as well as apoptosis impacts eIF5A-2 localization in various cellular compartments. eIF5A-2 exhibits weak expression levels in lungs, testis and brain cells, however, its elevated expression has been shown to be associated with the presence of cancer metastases in colon, ovarian, lung and bladder cancers. In vitro and in vivo studies have revealed that overexpression of eIF5A-2 could significantly enhance the malignant tumor cell motility/invasiveness, whereas, its intracellular depletion leads to inhibition of cell growth as well as induction of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF5A2 Antibody (NB200-133)(2)

Publications using NB200-133

• Clement PM, Johansson HE, Wolff EC, Park MH. Differential expression of eIF5A-1 and eIF5A-2 in human cancer cells. FEBS J 273(6):1102-14. 2006-03-01 [PMID: 16519677] (WB, Human)
• Clement, P. Identification Characterization of eukaryotic initiation factor 5A-2. Eur J Biochem 270, 4254-4263. 2003-01-01 [PMID: 14622290] (WB, Human)

Bioinformatics

• Gene Symbol: EIF5A2
• Entrez:
  • 56648(Human)