EIF4A3 Antibody

Images

 
Western Blot: EIF4A3 Antibody [NBP2-16305] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EIF4A3 Antibody [NBP2-16305] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.28 mg/ml

Order Details

EIF4A3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF4A3. The exact sequence is proprietary.
Localization
Nucleus; Nucleus speckle; Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (100%), Xenopus tropicalis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.28 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EIF4A3 Antibody

  • ATP-dependent RNA helicase DDX48
  • ATP-dependent RNA helicase eIF4A-3
  • DDX48
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 48
  • DEAD box protein 48
  • DKFZp686O16189
  • EC 3.6.1
  • EC 3.6.4.13
  • EIF4AIII
  • eIF-4A-III
  • eIF4A-III
  • eukaryotic initiation factor 4A-III
  • Eukaryotic initiation factor 4A-like NUK-34
  • Eukaryotic translation initiation factor 4A isoform 3
  • eukaryotic translation initiation factor 4A
  • eukaryotic translation initiation factor 4A3
  • hNMP 265
  • KIAA0111eukaryotic translation initiation factor 4A, isoform 3
  • MGC10862
  • NMP 265
  • NMP265
  • Nuclear matrix protein 265
  • NUK34

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF4A3
Uniprot