EIF4A3 Antibody

Western Blot: EIF4A3 Antibody [NBP2-16305] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: EIF4A3 Antibody [NBP2-16305] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Product Discontinued

EIF4A3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human EIF4A3. The exact sequence is proprietary.
• Localization: Nucleus; Nucleus speckle; Cytoplasm
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Zebrafish (99%), Rhesus Macaque (100%), Chicken (100%), Xenopus (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4A3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%), Xenopus laevis (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for EIF4A3 Antibody

• ATP-dependent RNA helicase DDX48
• ATP-dependent RNA helicase eIF4A-3
• DDX48
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 48
• DEAD box protein 48
• DKFZp686O16189
• EC 3.6.1
• EC 3.6.4.13
• eIF4AIII
• eIF-4A-III
• eIF4A-III
• eukaryotic initiation factor 4A-III
• Eukaryotic initiation factor 4A-like NUK-34
• Eukaryotic translation initiation factor 4A isoform 3
• eukaryotic translation initiation factor 4A
• eukaryotic translation initiation factor 4A3
• hNMP 265
• KIAA0111eukaryotic translation initiation factor 4A, isoform 3
• MGC10862
• NMP 265
• NMP265
• Nuclear matrix protein 265
• NUK34

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EIF4A3
• Uniprot:
  • P38919(Human)