EGR2 Antibody - BSA Free

Western Blot: EGR2 Antibody [NBP2-16286] - Expression in transfected 293T cell line. A: Non-transfected lysate. B: EGR2 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.

Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286] - Immunohistochemical analysis of mouse brain tissue (paraformaldehyde-fixed frozen sections), using antibody at 1:500 dilution.

Western Blot: EGR2 Antibody [NBP2-16286] - Sample (30 ug of whole cell lysate) A: zebrafish brain 10% SDS PAGE NBP2-16286 diluted at 1:1000

Immunohistochemistry: EGR2 Antibody [NBP2-16286] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using EGR2 antibody (NBP2-16286) at 1:300 dilution.

• Reactivity: Hu, Mu, Ze, Rt, Po, RM, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EGR2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human EGR2. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (90%), Porcine (95%), Rhesus Macaque (98%), Xenopus (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EGR2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:35151688) .
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for EGR2 Antibody - BSA Free

• AT591
• CMT1D
• CMT4E
• early growth response 2 (Krox-20 homolog, Drosophila)
• early growth response 2
• early growth response protein 2
• EGR-2
• FLJ14547
• Krox-20 homolog, Drosophila
• KROX-20, Drosophila, homolog (early growth response-2)
• KROX20DKFZp686J1957
• Zinc finger protein Krox-20

Background

The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for EGR2 Antibody (NBP2-16286)(1)

Publication using NBP2-16286

• Zhao Y, Liang Y, Xu Z et al. Exosomal miR-673-5p from fibroblasts promotes Schwann cell-mediated peripheral neuron myelination by targeting the TSC2/mTORC1/SREBP2 axis The Journal of biological chemistry 2022-02-10 [PMID: 35151688] (ICC/IF, Rat)

Bioinformatics

• Gene Symbol: EGR2
• Uniprot:
  • P11161(Human)