EGR2 Antibody (OTI1B12) - Azide and BSA Free

Western Blot: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGR2.

Immunocytochemistry/Immunofluorescence: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY EGR2.

Immunohistochemistry: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunocytochemistry/Immunofluorescence: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624] - Analysis of HeLa cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI1B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

EGR2 Antibody (OTI1B12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse EGR2 Antibody (OTI1B12) - Azide and BSA Free (NBP2-45696) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 156-476 of human EGR2(NP_000390) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EGR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 50.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for EGR2 Antibody (OTI1B12) - Azide and BSA Free

• AT591
• CMT1D
• CMT4E
• early growth response 2 (Krox-20 homolog, Drosophila)
• early growth response 2
• early growth response protein 2
• EGR-2
• FLJ14547
• Krox-20 homolog, Drosophila
• KROX-20, Drosophila, homolog (early growth response-2)
• KROX20DKFZp686J1957
• Zinc finger protein Krox-20

Background

Early Growth Response 2 (EGR2) is an early growth response family member which binds to specific areas of DNA and helps control the activity of particular genes. Specifically, EGR2 activates several genes that are involved in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells.

Defects in EGR2 have been linked to a number of diseases including congenital hypomyelination neuropathy, Charcot-Marie-Tooth disease type 1D, and Dejerine-Sottas neuropathy. EGR 2 antibodies may be useful tools for nerurscience research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EGR2