EGFLAM Overexpression Lysate (Native)

Images

 
Western Blot: EGFLAM Overexpression Lysate (Adult Normal) [NBP2-05379] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EGFLAM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EGFLAM Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_689616. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens EGFLAM, mRNA.
Gene
EGFLAM

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
110.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EGFLAM

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EGFLAM Overexpression Lysate (Native)

  • AGRINLAGRNLFLJ39155
  • Agrin-like protein
  • EGF-like, fibronectin type III and laminin G domains
  • EGF-like, fibronectin type-III and laminin G-like domain-containing protein
  • pikachurin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-14868
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-60655
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-12154
Species: Hu
NBP1-33084
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB120-7164
Species: Hu, Mu, Rt, Fi
Applications: ELISA, ICC/IF, IHC-Fr, MiAr
NBP1-93459
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-49865
Species: Hu
Applications: WB, PEP-ELISA
NBP1-81788
Species: Hu
Applications: IHC, IHC-P
AF1979
Species: Hu
Applications: WB, Simple Western, ICC
NBP2-38093
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87402
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-14001
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-22212
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-44405
Species: Hu
Applications: Flow, IHC-P, IF
MAB1233
Species: Hu
Applications: Flow, IP, CyTOF-ready, ICC
NBP2-05379
Species: Hu
Applications: WB

Publications for EGFLAM Lysate (NBP2-05379) (0)

There are no publications for EGFLAM Lysate (NBP2-05379).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EGFLAM Lysate (NBP2-05379) (0)

There are no reviews for EGFLAM Lysate (NBP2-05379). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EGFLAM Lysate (NBP2-05379) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EGFLAM Products

Bioinformatics Tool for EGFLAM Lysate (NBP2-05379)

Discover related pathways, diseases and genes to EGFLAM Lysate (NBP2-05379). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EGFLAM Lysate (NBP2-05379)

Discover more about diseases related to EGFLAM Lysate (NBP2-05379).
 

Pathways for EGFLAM Lysate (NBP2-05379)

View related products by pathway.

PTMs for EGFLAM Lysate (NBP2-05379)

Learn more about PTMs related to EGFLAM Lysate (NBP2-05379).

Blogs on EGFLAM

There are no specific blogs for EGFLAM, but you can read our latest blog posts.

Customers Who Bought This Also Bought

p19ARF Antibody
NB200-106
EGFLAM Antibody
NBP2-14941
FGF-10 [Unconjugated]
345-FG-025/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EGFLAM Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol EGFLAM