EEF1E1 Antibody - BSA Free

Western Blot: EEF1E1 Antibody - BSA Free [NBP3-46598] - HepG2 cells were subjected to SDS PAGE followed by western blot with (EEF1E1 Antibody) at dilution of 1:1000

Immunohistochemistry: EEF1E1 Antibody - BSA Free [NBP3-46598] - Immunohistochemistry of paraffin-embedded human breast cancer using (EEF1E1 antibody) at dilution of 1:10

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EEF1E1 Antibody - BSA Free Summary

• Immunogen: eukaryotic translation elongation factor 1 epsilon 1. (Uniprot# O43324)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEF1E1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for EEF1E1 Antibody - BSA Free

• AIMP3Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3
• ARS-interacting multifunctional protein 3
• Elongation factor p18
• eukaryotic translation elongation factor 1 epsilon 1
• eukaryotic translation elongation factor 1 epsilon-1
• Multisynthase complex auxiliary component p18
• p18 component of aminoacyl-tRNA synthetase complex
• P18

Background

EEF1E1 encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EEF1E1