eEF-2 Antibody [mFluor Violet 500 SE]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: mFluor Violet 500 SE

eEF-2 Antibody [mFluor Violet 500 SE] Summary

• Immunogen: E. coli-derived recombinant human eEF-2
  Val300-Ser500
  Accession # Q6PK56
• Specificity: Detects human, mouse, and rat eEF-2 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EEF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for eEF-2 Antibody [mFluor Violet 500 SE]

• eEF2
• eEF-2
• EF-2
• EF2EEF-2
• elongation factor 2
• eukaryotic translation elongation factor 2
• polypeptidyl-tRNA translocase

Background

The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EEF2