EDR1/PHC1 Overexpression Lysate (Native)

Images

 
Western Blot: EDR1/PHC1 Overexpression Lysate (Adult Normal) [NBL1-14350] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EDR1/PHC1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EDR1/PHC1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC222530 and based on accession number NM_004426. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens polyhomeotic homolog 1 (Drosophila) (PHC1), mRNA.
Gene
PHC1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
105.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EDR1/PHC1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EDR1/PHC1 Overexpression Lysate (Native)

  • early development regulator 1 (homolog of polyhomeotic 1)
  • Early development regulatory protein 1
  • EDR1
  • HPH1
  • PH1
  • polyhomeotic homolog 1 (Drosophila)
  • polyhomeotic-like 1 (Drosophila)
  • polyhomeotic-like 1
  • polyhomeotic-like protein 1
  • RAE28

Background

This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89200
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
NBP1-91979
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-03348
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-52084
Species: Hu, Mu, Rt
Applications: WB, IHC-P, PEP-ELISA
NBP1-33748
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-84896
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
21650002
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-37370
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready
NBP2-02300
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP2-47524
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00006045-M03
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
AF6394
Species: Hu
Applications: WB, IP
NBP1-31558
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
NB100-139
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-P, IP
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBL1-14350
Species: Hu
Applications: WB

Publications for EDR1/PHC1 Lysate (NBL1-14350) (0)

There are no publications for EDR1/PHC1 Lysate (NBL1-14350).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EDR1/PHC1 Lysate (NBL1-14350) (0)

There are no reviews for EDR1/PHC1 Lysate (NBL1-14350). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EDR1/PHC1 Lysate (NBL1-14350) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EDR1/PHC1 Products

Bioinformatics Tool for EDR1/PHC1 Lysate (NBL1-14350)

Discover related pathways, diseases and genes to EDR1/PHC1 Lysate (NBL1-14350). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EDR1/PHC1 Lysate (NBL1-14350)

Discover more about diseases related to EDR1/PHC1 Lysate (NBL1-14350).
 

Pathways for EDR1/PHC1 Lysate (NBL1-14350)

View related products by pathway.

PTMs for EDR1/PHC1 Lysate (NBL1-14350)

Learn more about PTMs related to EDR1/PHC1 Lysate (NBL1-14350).
 

Research Areas for EDR1/PHC1 Lysate (NBL1-14350)

Find related products by research area.

Blogs on EDR1/PHC1

There are no specific blogs for EDR1/PHC1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

p19ARF Antibody
NB200-106

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EDR1/PHC1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PHC1