ECRG4 Antibody (OTI3B10)

Western Blot: ECRG4 Antibody (3B10) [NBP2-00599] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECRG4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECRG4.

Immunocytochemistry/Immunofluorescence: ECRG4 Antibody (3B10) [NBP2-00599] - Immunofluorescent staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.

Flow Cytometry: ECRG4 Antibody (3B10) [NBP2-00599] - Analysis of Jurkat cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: ECRG4 Antibody (3B10) [NBP2-00599] - Analysis of Hela cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: OTI3B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.85 mg/ml

ECRG4 Antibody (OTI3B10) Summary

• Immunogen: Full length human recombinant protein of human C2orf40 (NP_115787) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: C2orf40
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.85 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ECRG4 Antibody (OTI3B10)

• chromosome 2 open reading frame 40
• ECRG4augurin
• esophageal cancer related gene 4 protein
• Esophageal cancer-related gene 4 protein

Background

ECRG4, also known as C2orf40 or Chromosome 2 Open Reading Frame 40, is a secreted hormone that may stimulate senescence of neural precursor cells and oligodendrocytes. Current research on ECRG4is being performed in relation to several diseases and disorders including esophageal cancer, esophagitis and breast cancer. ECRG4 has also been shown to interact with p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ECRG4 Antibody (NBP2-00599)(1)

Publication using NBP2-00599

• Huang W, Zhou R, Mao L, Deng C et al. Esophageal cancer related gene-4 inhibits the migration and proliferation of oral squamous cell carcinoma through BC200 lncRNA/MMP-9 and -13 signaling pathway Cellular Signalling 2019-05-01 [PMID: 31152845]

Bioinformatics

• Gene Symbol: C2orf40
• Entrez:
  • 84417(Human)