E2F6 Antibody (1F7-A9)

Images

 
Western Blot: E2F6 Antibody (1F7-A9) [H00001876-M02] - Western Blot analysis of E2F6 expression in A-549 ( Cat # L025V1 ).
Immunocytochemistry/ Immunofluorescence: E2F6 Antibody (1F7-A9) [H00001876-M02] - Analysis of monoclonal antibody to E2F6 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: E2F6 Antibody (1F7-A9) [H00001876-M02] - Western Blot analysis of E2F6 expression in PC-12(Cat # L012V1 ).
Western Blot: E2F6 Antibody (1F7-A9) [H00001876-M02] - Western Blot analysis of E2F6 expression in HepG2 ( Cat # L019V1 ).
Sandwich ELISA: E2F6 Antibody (1F7-A9) [H00001876-M02] - Detection limit for recombinant GST tagged E2F6 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, S-ELISA
Clone
1F7-A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

E2F6 Antibody (1F7-A9) Summary

Immunogen
E2F6 (AAH08348, 1 a.a. ~ 281 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSQQRPARKLPSLLLDPTEETVRRRCRDPINVEGLLPSKIRINLEDNVQYVSMRKALKVKRPRFDVSLVYLTRKFMDLVRSAPGGILDLNKVATKLGVRKRRVYDITNVLDGIDLVEKKSKNHIRWIGSDLSNFGAVPQQKKLQEELSDLSAMEDALDELIKDCAQQLFELTDDKENERLAYVTYQDIHSIQAFHEQIVIAVKAPAETRLDVPAPREDSITVHIRSTNGPIDVYLCEVEQGQTSNKRSEGVGTSSSESTHPEGPEEEENPQQSEELLEVSN
Specificity
E2F6 - E2F transcription factor 6
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
E2F6
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for E2F6 Antibody (1F7-A9)

  • E2F transcription factor 6
  • E2F-6MGC111545
  • transcription factor E2F6

Background

This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol E2F6
Entrez
OMIM
Uniprot