E2F-1 Antibody

Product Discontinued

E2F-1 Antibody Summary

• Immunogen: Synthetic peptide: KTPSQEVTSEEENRATDSAT conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 310-329 of Human E2F 1.
• Localization: Nuclear
• Specificity: E2F1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: E2F1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2.5 ug/ml
• Application Notes: Immunoprecipitation, Western Blot and Microarray: In Western BLot a 50kDa band is seen. Optimal dilutions should be determined by the end user.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for E2F-1 Antibody

• E2F transcription factor 1
• E2F1
• E2F-1
• PBR3
• PRB-binding protein E2F-1
• RBAP1
• RBAP-1
• RBBP3
• RBBP-3
• RBP3
• Retinoblastoma-associated protein 1
• Retinoblastoma-binding protein 3
• transcription factor E2F1

Background

Anti-E2F1 reacts specifically with the E2F1 transcription regulator protein (recombinant). The antibody may be used for immunoblotting (~50 kDa) and immunoprecipitation techniques using whole cell extracts of E2F1 transfected cells, or in vitro translated E2F1 proteins. Staining of the E2F1 band by immunoblotting is specifically inhibited with the immunizing E2F1 peptide. The E2F family of transcription factors is important for cell cycle control. The E2F transcription factors are heterodimeric molecules composed of two distinct proteins termed E2F and DP. The E2F component of this protein complex is encoded by a family of genes consisting of at least 6 members (E2F-1 to -6). The DP family contains three proteins (DP -1 to -3), which heterodimerize interchangeably with any of the E2F proteins. E2F/DP target genes include genes important for DNA replication (e.g. DHFR, ORC1, MCM), cyclin genes (A and E), proto-oncogene (myc, myb) and the Rb family genes (Rb, p107). The E2F/DP transcription factors are tightly regulated by multiple regulatory mechanisms. Besides being regulated by the combination of the two components, the E2F/DP complex is negatively regulated by binding to one or more members of the retinoblastoma (Rb) pocket protein family: pRb, p107 and p130. Complexes of unphosphorylated pRb and E2F/DP act as transcriptional repressors that contribute to the Rbdependent G1 arrest. Once a pocket protein is phosphorylated by CDKs, the E2F/DP/pocket protein complex dissociates, resulting in free, transcriptionally active E2F/DP heterodimer. Another level of regulation is the synthesis and degradation of the proteins. Certain E2F species are actively degraded by the ubiquitin-proteasome pathway and their synthesis is cell-cycle regulated. The E2F proteins can be grouped into 3 subfamilies based upon structural homology and functional characteristics. The first subfamily consists of E2F-1, -2, and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: E2F1
• Entrez:
  • 1869(Human)