DUSP8 Antibody

Western Blot: DUSP8 Antibody [NB100-56410] - Analysis of DUSP8 in HeLa cell lysate.

Product Discontinued

DUSP8 Antibody Summary

• Immunogen: A portion of amino acids 250-300 of human DUSP8 were used as the immunogen.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DUSP8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Reacts with Human. Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for DUSP8 Antibody

• C11orf81
• chromosome 11 open reading frame 81
• dual specificity phosphatase 8
• Dual specificity protein phosphatase hVH-5
• EC 3.1.3.16
• EC 3.1.3.48
• FLJ42476
• FLJ42958
• HVH8
• serine/threonine specific protein phosphatase
• vaccinia virus homolog
• VH5

Background

Ser/Thr and Tyr dual specificity phosphatases comprise a group of enzymes with both Ser/Thr and tyrosine specific protein phosphatase activity which are capable of removing both the serine/threonine or tyrosine-bound phosphate groups from a wide range of phosphoproteins, which have been phosphorylated under the action of a kinase. Dual specificity protein phosphatases (DSPs) are implicated in the roles of mitogenic signal transduction regulation as well as cell cycle control. The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress as well as growth factors and specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family. It has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. Purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Additionally, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. The Dual specificity protein phosphatase motif is found in 5 isoforms from this gene. 45 other genes in the database also contain this motif.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUSP8 Antibody (NB100-56410)(1)

Publication using NB100-56410

• Qiao X, Zhu Y, Dang W et al. Dual-specificity phosphatase 15 (DUSP15) in the nucleus accumbens is a novel negative regulator of morphine-associated contextual memory Addict Biol 2020-02-11 [PMID: 32043707]

Bioinformatics

• Gene Symbol: DUSP8
• Entrez:
  • 1850(Human)
  • 13202(Mouse)