DUSP5 Overexpression Lysate (Native)

Images

 
Western Blot: DUSP5 Overexpression Lysate (Adult Normal) [NBL1-10056] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DUSP5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DUSP5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209128 and based on accession number NM_004419. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens dual specificity phosphatase 5 (DUSP5), mRNA.
Gene
DUSP5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DUSP5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DUSP5 Overexpression Lysate (Native)

  • dual specificity phosphatase 5
  • dual specificity protein phosphatase 5
  • Dual specificity protein phosphatase hVH3
  • DUSP
  • EC 3.1.3.16
  • EC 3.1.3.48
  • HVH3VH1-like phosphatase 3
  • serine/threonine specific protein phosphatase
  • VH3

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-834
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-34252
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NBP1-86613
Species: Hu
Applications: WB, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
H00001848-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
H00011266-D01P
Species: Hu
Applications: WB, ELISA, ICC/IF
H00001846-M08
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-01340
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF
AF1347
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
AF3720
Species: Hu, Mu
Applications: WB, ICC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-80073
Species: Hu, Rt
Applications: WB, IHC, IHC-P
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBL1-10056
Species: Hu
Applications: WB

Publications for DUSP5 Lysate (NBL1-10056) (0)

There are no publications for DUSP5 Lysate (NBL1-10056).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DUSP5 Lysate (NBL1-10056) (0)

There are no reviews for DUSP5 Lysate (NBL1-10056). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DUSP5 Lysate (NBL1-10056) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DUSP5 Products

Bioinformatics Tool for DUSP5 Lysate (NBL1-10056)

Discover related pathways, diseases and genes to DUSP5 Lysate (NBL1-10056). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DUSP5 Lysate (NBL1-10056)

Discover more about diseases related to DUSP5 Lysate (NBL1-10056).
 

Pathways for DUSP5 Lysate (NBL1-10056)

View related products by pathway.

PTMs for DUSP5 Lysate (NBL1-10056)

Learn more about PTMs related to DUSP5 Lysate (NBL1-10056).
 

Research Areas for DUSP5 Lysate (NBL1-10056)

Find related products by research area.

Blogs on DUSP5

There are no specific blogs for DUSP5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-2 [Unconjugated]
202-IL-010/CF
DUSP5 Antibody (2F3)
H00001847-M04

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DUSP5 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DUSP5