Recombinant Human DUSP10 Protein

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Recombinant Human DUSP10 Protein [NBP1-78891]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
0.25 mg/ml

Order Details

Recombinant Human DUSP10 Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 149-482 of Human DUSP10

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMIIYPN DLAKKMTKCS KSHLPSQGPV IIDCRPFMEY NKSHIQGAVH INCADKISRR RLQQGKITVL DLISCREGKD SFKRIFSKEI IVYDENTNEP SRVMPSQPLH IVLESLKREG KEPLVLKGGL SSFKQNHENL CDNSLQLQEC REVGGGASAA SSLLPQPIPT TPDIENAELT PILPFLFLGN EQDAQDLDTM QRLNIGYVIN VTTHLPLYHY EKGLFNYKRL PATDSNKQNL RQYFEEAFEF IEEAHQCGKG LLIHCQAGVS RSATIVIAYL MKHTRMTMTD AYKFVKGKRP IISPNLNFMG QLLEFEEDLN NGVTPRILTP KLMGVETVV

Details of Functionality
Specific activity is > 600 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
DUSP10
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200mM NaCl
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human DUSP10 Protein

  • dual specificity phosphatase 10
  • dual specificity phosphatase MKP-5
  • EC 3.1.3.16
  • EC 3.1.3.48
  • MAP kinase phosphatase 5
  • Mitogen-activated protein kinase phosphatase 5
  • MKP-5dual specificity protein phosphatase 10
  • MKP5serine/threonine specific protein phosphatase

Background

DUSP10 (Dual specificity protein phosphatase 10) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases(DUSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. DUSP10 has been shown to interact with MAPK14 and MAPK8. When expressed in mammalian cells, DUSP10 blocks the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK. Recombinant human DUSP10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol DUSP10