Draxin/C1orf187 Antibody [Unconjugated]

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Draxin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Draxin was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6148) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to diencephalon. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Draxin/C1orf187 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Draxin
  aa 26-349
  Accession # Q8NBI3
• Specificity: Detects human Draxin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Draxin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DRAXIN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Draxin/C1orf187 Antibody [Unconjugated]

• AGPA3119
• C1orf187
• chromosome 1 open reading frame 187
• dorsal inhibitory axon guidance protein
• Dorsal repulsive axon guidance protein
• Draxin
• FLJ34999
• MGC117222
• Neucrin
• UNQ3119

Background

Draxin (Dorsal repulsive axon guidance protein; also neucrin) is a secreted, 58 kDa, presumable glycoprotein member of the draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature human Draxin is 324 amino acids (aa) in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa 274-333). Mature human Draxin (aa 26-349) shares 79% aa identity with mature mouse Draxin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Draxin/C1orf187 Antibody (AF6148)(1)

Publication using AF6148

• Tianxiang Huang, Lena H. Nguyen, Tiffany V. Lin, Xuan Gong, Longbo Zhang, Gi Bum Kim et al. In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons Neuroscience Research 2019-06-01 [PMID: 29857015] (Western Blot, Mouse)

Review for Draxin/C1orf187 Antibody (AF6148) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer IF Other 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23038776
• Species: Other

Bioinformatics

• Gene Symbol: DRAXIN
• Uniprot:
  • Q8NBI3(Human)