DPYSL4 Antibody Blocking Peptide

• Reactivity: Hu
• Applications: AC
• Concentration: 2.5 mg/ml

DPYSL4 Antibody Blocking Peptide Summary

• Description: Synthetic peptide corresponding to amino acids 509-527 of human CRMP3.
  
  NCBI Accession #NP_006417.2

  Source: Synthetic

  Amino Acid Sequence: PAKPGSGTQARASCSGKIS

• Source: Synthetic
• Protein/Peptide Type: Antibody Blocking Peptide
• Gene: DPYSL4
• Purity: >85%, by HPLC

Applications/Dilutions

• Dilutions:
  • Antibody Competition
• Application Notes: This peptide is useful as a blocking peptide for NBP2-11055.
  
  For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Sodium Bicarbonate buffer, pH 8.00 containing up to 10% DMSO
• Concentration: 2.5 mg/ml
• Purity: >85%, by HPLC

Alternate Names for DPYSL4 Antibody Blocking Peptide

• CRMP-3
• CRMP3Collapsin response mediator protein 3
• dihydropyrimidinase-like 4
• dihydropyrimidinase-related protein 4
• DRP-4UNC33-like phosphoprotein 4
• ULIP4ULIP-4

Background

Collapsin response mediator proteins (CRMPs) specify axon/dendrite fate and axonal growth of neurons through protein-protein interactions. Collapsing mediated response proteins (CRMPs) form homo- and hetero tetramers and facilitate neuron guidance, growth and polarity. It is abundantly expressed in brain and regulate neurite outgrowth. The CRMP family consists of 5 subtypes (CRMP1-5) and are also known as dihydroxypyrimindinase 1-5 (DRP1-5). The CRMPs promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse and also plays a role in synaptic signaling through interactions with calcium channels. CRMP2 protein regulates endocytosis where two proteins MICAL-L1 and EHD1 are important regulatory proteins that control key endocytotic transport steps. CRMP2 interacts with MICAL-L1 in non-neuronal cells (1). CRMP2 interacts with tubulin dimers and kinesin and negatively regulates s dynein-based transport in neuronal cells (2). The mammalian target of rapamycin (mTOR)is an important regulator of neuronal development and function. mTOR inhibitor, rapamycin regulates neuronal polarity by suppressing the expression of Tau and CRMP2 via mTOR effector 70-kDa ribosomal protein S6 kinase (p70S6K)p70 (2). It is suggested that abnormal activation of CDK5 is associated with neurodegeneratative disorders and it disrupts mature neuronal circuitries and neurogenesis. CDK5 and other kinases hyper-phosphorylate down-stream targets of these kinases in brain such as doublecortin, nestin, and CRMP2 (2). This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. DRP4-selective antibodies were generated against peptides form unique region near C-terminus of DRP4 protein with in aa 508-527. The epitope is conserved in several other species beside human but was unique to DRP4. The DRP4 antibodies are affinity purified over immobilized antigen based affinity chromatography and fully characterized for their listed applications and cross reactivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DPYSL4