DPP10 Antibody

Western Blot: DPP10 Antibody [NB100-1511] - Analysis of Human Bone Marrow lysate (35ug protein in RIPA buffer) using this antibody 0.1ug/ml.

Immunohistochemistry-Paraffin: DPP10 Antibody [NB100-1511] - staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: DPP10 Antibody [NB100-1511] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DPP10 Antibody Summary

• Description: Novus Biologicals Goat DPP10 Antibody (NB100-1511) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DEGHNVSEKSKYHL corresponding to internal region according to NP_065919.2, NP_001004360.2.
• Specificity: This antibody is expected to recognise both reported isoforms NP_065919.2 and NP_001004360.2.
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DPP10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human bone marrow lysates (calculated MW of 90.0 kDa band according to NP_065919.2 and of 90.1 according to NP_001004360.1).
  IHC: Paraffin embedded Human Brain (Cortex and Cerebellum).
• Control:

  DPP10 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DPP10 Antibody

• dipeptidyl peptidase 10
• Dipeptidyl peptidase IV-related protein 3
• dipeptidyl peptidase like protein 2
• Dipeptidyl peptidase X
• Dipeptidyl peptidase-like protein 2
• dipeptidyl-peptidase 10 (non-functional)
• dipeptidyl-peptidase 10
• DPL2
• DPP X
• DPP10
• DPPY
• DPRP3
• DPRP-3
• DPRP3dipeptidylpeptidase 10
• inactive dipeptidyl peptidase 10
• KIAA1492

Background

A novel gene, DPP10, encodes a homolog of dipeptidyl peptidases (DPPs) that cleave terminal dipeptides from cytokines and chemokines, and it presents a potential new target for asthma therapy. The DPP10 protein shares features with members of the S9B family of DPP serine proteases, which includes DPP4, a widely expressed enzyme that plays a central role in chemokine processing as part of the innate immune system. The locus displays a complex pattern of transcript splicing, with eight alternate first exons; four of which localize within the LD block strongly associated with asthma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPP10
• Entrez:
  • 57628(Human)
• Uniprot:
  • NP_065919.2()