Recombinant Human DPH4 His Protein

SDS-Page: DPH4 Protein [NBP2-23007]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human DPH4 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-149 of Human DPH4
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMAVEQM PKKDWYSILG ADPSANISDL KQKYQKLILM YHPDKQSTDV PAGTVEECVQ KFIEIDQAWK ILGNEETKRE YDLQRCEDDL RNVGPVDAQV YLEEMSWNEG DHSFYLSCRC GGKYSVSKDE AEEVSLISCD TCSLIIELLH YN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DNAJC24
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 19.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human DPH4 His Protein

• CSL-type zinc finger-containing protein 3
• DnaJ (Hsp40) homolog, subfamily C, member 24
• dnaJ homolog subfamily C member 24
• DPH4 homolog
• DPH4, JJJ3 homolog (S. cerevisiae)
• DPH4, JJJ3 homolog
• DPH41700030A21Rik
• JJJ3
• S. cerevisiae)
• ZCSL3
• zinc finger, CSL domain containing 3
• zinc finger, CSL-type containing 3

Background

DnaJ homolog subfamily C member 24, also known as DNAJC24, is a member of the ZCSL family of proteins and each contain one DPH-type zinc finger. This enzyme is involved in the synthesis of diphthamide, a protein found on translation elongation factor EF-2 that is the target of bacterial ADP-ribosylating toxins. DNAJC24 stimulates the ATPase activity of several Hsp70-type chaperones. It is detected in heart, brain, spleen, lung, liver, kidney and testis. Recombinant human DNAJC24 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DNAJC24