DPF3 Antibody

Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Analysis in human cerebral cortex and prostate tissues using NBP2-49494 antibody. Corresponding DPF3 RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: DPF3 Antibody [NBP2-49494] - Staining of human cell line BJ shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Analysis in human cerebral cortex and prostate tissues using NBP2-49494 antibody. Corresponding DPF3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: DPF3 Antibody [NBP2-49494] -Staining of human prostate shows no positivity in glandular cells as expected.

Product Discontinued

DPF3 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: KLRLLEIKPEVELPLKKDGFTSESTTLEALLRGEGVEKKVDAREEESIQEIQRVLENDENVEEGNEEEDLEEDIPKRKNRTRGRA
• Predicted Species: Mouse (99%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DPF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DPF3 Antibody

• CERD4
• D4, zinc and double PHD fingers, family 3

Background

Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPF3