DOG1/TMEM16A Antibody (SPM580)

Immunocytochemistry/Immunofluorescence: DOG1/TMEM16A Antibody (SPM580) [NBP2-32841] - HEK cells expressing Ano1-Myc. SPM580 gave more signal than the Myc Ab control and much more than the SP31 mAb. Negative control cells were negative. ICC image submitted by a verified customer review.

Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (SPM580) [NBP2-32841] - Formalin-fixed paraffin-embedded human GIST stained with DOG1 Monoclonal Antibody (SPM580).

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: SPM580
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DOG1/TMEM16A Antibody (SPM580) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34812)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human canine1/TMEM16A protein (Uniprot: Q5XX6)
• Localization: Cell Surface and Cytoplasm
• Marker: Marker for Gastrointestinal Stromal Tumors
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANO1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 0.5-1.0ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Validated for ICC/IF & WB from a verified customer review.
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for DOG1/TMEM16A Antibody (SPM580)

• ANO1
• anoctamin 1
• DOG1
• ORAOV2
• TAOS2
• TMEM16A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for DOG1/TMEM16A Antibody (NBP2-32841) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, MDCK.

reviewed by: Verified Customer WB MDCK 11/27/2019

Summary

• Application: Western Blot
• Sample Tested: MDCK kidney protein lysate,protein lysates
• Species: MDCK
• Lot: 160108LVF

Comments

• Comments: 50µg Protein load on normal SDS gel
  20 min at 70V and then 1h at 120V
  Tank blotting for 3h at 120V in PVDF membrane
  Blocking for 1h with 5%MM/TBST
  Prim. AB 1:500 in 1%MM/TBST over night

reviewed by: Randolph Elble ICC Human 02/01/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Hek 293T
• Species: Human

Bioinformatics

• Gene Symbol: ANO1
• Uniprot:
  • Q5XXA6(Human)