DOCK9 Antibody - BSA Free

Western Blot: DOCK9 Antibody [NB500-265] - Expression profile of Zizimin1 and Zizimin2 protein. Western blot analysis shows the expression levels of Zizimin1 and Zizimin2 in murine tissues described at the top of the panels. alpha-Tubulin was used as a loading control. Image collected and cropped by CiteAb from the following publication (immunityageing.biomedcentral.com/articles/10.1186/1742-4933-9-2), licensed under a CC-BY license.

Western Blot: DOCK9 Antibody [NB500-265] - Detection of Human and Mouse DOCK9 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DOCK9 antibody NB500-265 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: DOCK9 Antibody [NB500-265] - Zizimin2, Zizimin1, and Zizimin3 expression in young and aged mice. Lysates were prepared from three individual three young (7-8 weeks old) or aged (24 months old) mouse tissues, which are described to the left of each panel, followed by western blotting. alpha-Tubulin was used as a loading control. Image collected and cropped by CiteAb from the following publication (immunityageing.biomedcentral.com/articles/10.1186/1742-4933-9-2), licensed under a CC-BY license.

Immunoprecipitation: DOCK9 Antibody [NB500-265] - Detection of human DOCK9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK9 antibody NB500-265 (lot NB500-265-2) used for IP at 6 ug per reaction. DOCK9 was also immunoprecipitated by a previous lot of this antibody (lot NB500-265-1) and rabbit anti-DOCK9 antibodies NB500-263 and NB500-264. Detection: Chemiluminescence with an exposure time of 3 minutes.

Absence of Zizimin proteins, but normal numbers of hematopoietic cells in KO mice. (A-B) Western blotting for Ziz2 (A) or Ziz3 (B) Protein lysates (30 μg/lane) were loaded & designated antibodies were reacted with the membranes. Replicated membranes were used for all antibodies. Zizimin proteins were absent in KO mice (8 weeks old, male). (C-F) The numbers of hematopoietic cells were counted after the hemolytic reaction (C-E) or flow cytometric analysis (F). No significant difference was observed among the groups (C-F). Three mice (8 weeks old, female) per group from three independent experiments (one mouse per group per experiment) were used. 2KO: Ziz2 KO. 3KO: Ziz3 KO. Image collected & cropped by CiteAb from the following publication (//immunityageing.biomedcentral.com/articles/10.1186/s12979-015-0028-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DOCK9 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1850 and 1900 of human Dedicator of Cytokinesis 9 using the numbering given in entry NP_056111.1 (GeneID 23348).
• Localization: Cytoplasmic (membrane associated)
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DOCK9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:500-1:2500
• Theoretical MW: 236 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DOCK9 Antibody - BSA Free

• Cdc42 guanine nucleotide exchange factor zizimin-1
• dedicator of cytokinesis 9
• dedicator of cytokinesis protein 9
• DKFZp686C11110
• DKFZp686D2047
• DKFZp686N04132
• FLJ11949
• FLJ16744
• FLJ45601
• KIAA1058FLJ44528
• KIAA1085
• ZIZ1FLJ45282
• ZIZIMIN1

Background

DOCK9 is a guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DOCK9 Antibody (NB500-265)(2)

Publications using NB500-265

• Matsuda T, Yanase S, Takaoka A, Maruyama M. The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation Immun Ageing. 2015-03-02 [PMID: 25729399] (WB, Mouse)
• Sakabe I, Asai A, Iijima J, Maruyama M. Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells. Immun Ageing;9:2. 2012-04-11 [PMID: 22494997] (ICC/IF, WB, Mouse)

FAQs for DOCK9 Antibody (NB500-265). (Showing 1 - 1 of 1 FAQs).

1. The homology of DOCK9 and DOCK10 is high. Do the following antibodies cross-react with other DOCK family proteins? DOCK9 Antibody (NB500-265) and DOCK10 Antibody (NB100-60669).
   • I did a blast of the entire DOCK9 and DOCK10 sequences and they only have 50.54% identity between the two. In the amino acid ranges of the specific products NB500-265 and NB100-60669, I do not see any high similarity and would not predict these two proteins to cross react. We have not tested these products for cross reactivity with other DOCK proteins and cannot say with certainty that they will not cross react. Based on homology, I think it is unlikely.

Bioinformatics

• Gene Symbol: DOCK9
• Entrez:
  • 23348(Human)
• Uniprot:
  • Q9BZ29(Human)