Doc2-alpha Antibody (842213) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: IHC
• Clone: 842213
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Doc2-alpha Antibody (842213) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Doc2α
  Met1-Lys114
  Accession # Q14183
• Specificity: Detects human Doc2 alpha in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Doc2 beta is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Doc2-alpha Antibody (842213) [Alexa Fluor® 647]

• Doc2 alpha
• Doc2
• DOC2A
• doc2-alpha
• double C2-like domain-containing protein alpha
• double C2-like domains, alpha

Background

DOC2A/Doc2 alpha (Double C2-like domain containing protein alpha) is a 44 kDa (predicted) monomeric member of the C2 domain-containing protein family of molecules. It is expressed in both neurons and mast cells and appears to serve as an intracellular Ca⁺⁺ sensor protein that regulates secretory vesicle release. In neurons, Doc2 alpha is normally bound to synaptic vesicles and interacts with Munc13-1 to promote secretory vesicle exocytosis through the cell membrane. In mast cells, a similar process occurs that involves Munc13-4 instead of Munc13-1. Human Doc2 alpha is 400 amino acids (aa) in length. It contains a Mid domain (aa 13-37) that binds Munc13‑1, followed by one C2 domain that binds Ca⁺⁺ and lipid (aa 91-195) and a second C2 domain that binds SNAP25 (253-356). There is one potential alternative start site 16 aa upstream of the standard site. Over aa 1-114, human Doc2 alpha shares 90% aa sequence identity with mouse Doc2 alpha . Human DOC2B is the product of a separate gene and shares no meaningful aa sequence identity (<30%) with human Doc2 alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.