DNA Polymerase iota Antibody

Product Discontinued

DNA Polymerase iota Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 690 and 715 of human DNA polymerase iota using the numbering given in entry NP_009126.1 (GeneID 11201).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLI
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA Polymerase iota Antibody

• DNA polymerase iota
• EC 2.7.7.7
• eta2
• polymerase (DNA directed) iota
• RAD30 homolog B
• RAD30Bpolymerase (DNA-directed), iota
• RAD3OB

Background

Pol Iota is a DNA polymerase involved in DNA repair and translesion DNA synthesis. Pol Iota is a member of the Rad30 family of DNA polymerases which exhibit low fidelity when replicating undamaged DNA, and in this context may play a role in the hypermutation of immunoglobulin genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Polymerase iota Antibody (NB100-60668)(1)

Publication using NB100-60668

• Ziv O, Zeisel A, Mirlas-Neisberg N et al. Identification of novel DnA-damage tolerance genes reveals regulation of translesion DnA synthesis by nucleophosmin. Nat Commun. 2014-11-25 [PMID: 25421715] (WB, Human)

Bioinformatics

• Gene Symbol: POLI
• Entrez:
  • 11201(Human)
• Uniprot:
  • Q9UNA4(Human)