DNA Polymerase iota Antibody

Western Blot: DNA Polymerase iota Antibody [NB100-175] - Western blot analysis of DNA Polymerase iota in NIH-3T3 whole cell lysate.

Immunocytochemistry/Immunofluorescence: DNA Polymerase iota Antibody [NB100-175] - The DNA Polymerase Iota Antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

Immunoprecipitation: DNA Polymerase iota Antibody [NB100-175] - Characterization of DNA Polymerase iota acetylation. Effect of a p300/CBP inhibitor, L002, on MMS-induced DNA Polymerase iota acetylation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30886224/) licensed under a CC-BY license.

Product Discontinued

DNA Polymerase iota Antibody Summary

• Immunogen: A synthetic peptide made to the C-terminal region of human DNA Polymerase iota between amino acids 700-740, conjugated to KLH. [UniProt# Q9UNA4]
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLI
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: This DNA Polymerase iota antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~80 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for DNA Polymerase iota Antibody

• DNA polymerase iota
• EC 2.7.7.7
• eta2
• polymerase (DNA directed) iota
• RAD30 homolog B
• RAD30Bpolymerase (DNA-directed), iota
• RAD3OB

Background

DNA polymerase Iota (pol iota, also known as Rad30B) is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in poli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Polymerase iota Antibody (NB100-175)(4)

Publications using NB100-175

• McIntyre J, Sobolewska A, Fedorowicz M et al. DNA polymerase is acetylated in response to SN2 alkylating agents Sci Rep 2019-03-18 [PMID: 30886224] (WB, Human)
• Diamant N, Hendel A, Vered I,et al. DNA damage bypass operates in the S and G2 phases of the cell cycle and exhibits differential mutagenicity. Nucleic Acids Res. 2011-09-09 [PMID: 21908406] (WB, Human)
• Ziv O, Geacintov N, Nakajima S et al. DNA polymerase {zeta} cooperates with polymerases {kappa} {iota} in translesion DNA synthesis across pyrimidine photodimers in cells from XPV patients. PNAS;106(28):11552-11557. 2009-01-01 [PMID: 19564618]
• Choi JH, Besaratinia A, Lee DH et al. The role of DNA polymerase iota in UV mutational spectra. Mutat Res 2006-07-25 [PMID: 16472831] (Human)

Bioinformatics

• Gene Symbol: POLI
• Entrez:
  • 11201(Human)
• Uniprot:
  • Q9UNA4(Human)