DNA Polymerase iota Antibody

Images

 
Western Blot: DNA Polymerase iota Antibody [NB100-175] - Western blot analysis of DNA Polymerase iota in NIH-3T3 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase iota Antibody [NB100-175] - The DNA Polymerase Iota Antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DNA Polymerase iota Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of human DNA Polymerase iota between amino acids 700-740, conjugated to KLH. [UniProt# Q9UNA4]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLI
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:250
  • Western Blot 1:1000
Application Notes
This DNA Polymerase iota antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~80 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-175 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for DNA Polymerase iota Antibody

  • DNA polymerase iota
  • EC 2.7.7.7
  • eta2
  • polymerase (DNA directed) iota
  • RAD30 homolog B
  • RAD30Bpolymerase (DNA-directed), iota
  • RAD3OB

Background

DNA polymerase Iota (pol iota, also known as Rad30B) is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in poli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA Polymerase iota Antibody (NB100-175)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol POLI
Entrez
Uniprot