DNA Polymerase iota Antibody (OTI3B5) [Janelia Fluor® 646]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI3B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

DNA Polymerase iota Antibody (OTI3B5) [Janelia Fluor® 646] Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 458-740 of human POLI (NP_009126) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DNA Polymerase iota
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: >95%

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for DNA Polymerase iota Antibody (OTI3B5) [Janelia Fluor® 646]

• DNA polymerase iota
• EC 2.7.7.7
• eta2
• polymerase (DNA directed) iota
• RAD30 homolog B
• RAD30Bpolymerase (DNA-directed), iota
• RAD3OB

Background

DNA polymerase Iota (POLI), also known as Rad30B is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in DNA Pol I.

DNA Pol I antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNA Polymerase iota