DNA Polymerase iota Antibody (8G9)

Product Discontinued

DNA Polymerase iota Antibody (8G9) Summary

• Immunogen: POLI (AAH32662, 616 a.a. ~ 716 a.a) partial recombinant protein with GST tag.PQGFHFTNSNPAVSAFHSFPNLQSEQLFSRNHTTDSHKQTVA TDSHEGLTENREPDSVDEKITFPSDIDPQVFYELPEAVQKEL LAEWKRAGSDFHIGHK
• Localization: Nuclear
• Specificity: POLI - polymerase (DNA directed) iota
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLI
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNA Polymerase iota Antibody (8G9)

• DNA polymerase iota
• EC 2.7.7.7
• eta2
• polymerase (DNA directed) iota
• RAD30 homolog B
• RAD30Bpolymerase (DNA-directed), iota
• RAD3OB

Background

DNA Polymerase Iota, also known as Rad30B, is a novel human polymerase, unlike any others known. It is an extremely low fidelity enzyme that is highly error prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in DNA Polymerase Iota.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Polymerase iota Antibody (H00011201-M01)(3)

Publications using H00011201-M01

• Sekimoto T, Oda T, Kurashima K et al. Both High-fidelity Replicative and Low-fidelity Y-family Polymerases are Involved in DNA Rereplication. Mol Cell Biol. 2014-12-08 [PMID: 25487575]
• Takezawa J, Ishimi Y, Aiba N, Yamada K. Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation. J Nucleic Acids. 2010-12-01 [PMID: 21151666]
• Wang Y, Woodgate R, McManus TP et al. Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations. Cancer Res. 2007-04-01 [PMID: 17409408]

Bioinformatics

• Gene Symbol: POLI
• Entrez:
  • 11201(Human)
• OMIM:
  • 605252(Human)
• Uniprot:
  • AAH32662(Human)