DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-3D3)

Immunocytochemistry/Immunofluorescence: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-3D3) [NBP3-13740] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-3D3) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-3D3) [NBP3-13740] - Flow cytometric analysis of PFA-fixed HeLa cells. DNA Polymerase epsilon subunit 3 antibody (PCRP-POLE3-3D3) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-3D3) [NBP3-13740] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-3D3).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-POLE3-3D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-3D3) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14143)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human DNA Polymerase epsilon subunit 3 protein (Uniprot: Q9NRF9)
• Localization: Nucleus
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLE3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 16.86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Mouse, Rat and Xenopus.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-3D3)

• Arsenic-transactivated protein
• AsTP
• CHARAC17arsenic transactivated protein
• CHRAC-17
• CHRAC17Ybl1
• Chromatin accessibility complex 17 kDa protein
• DNA polymerase epsilon p17 subunit
• DNA polymerase epsilon subunit 3
• DNA polymerase epsilon subunit p17
• DNA polymerase II subunit 3
• EC 2.7.7.7
• histone fold protein CHRAC17
• huCHRAC17
• p17
• polymerase (DNA directed), epsilon 3 (p17 subunit)
• YBL1

Background

DNA polymerase epsilon (POLE) is an enzyme that helps catalyze in the polymerization of deoxyribonucleotides into a DNA strand. The C-terminus of DNA polymerase E subunit complexes with subunits B and C, whereas the N-terminal domain contains an active polymerase domain.

DNA Pol E antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLE3
• Uniprot:
  • Q9NRF9(Human)