DMP-1 Antibody

Western Blot: DMP-1 Antibody [NBP1-45525] - Odonto/osteogenic differentiation in canonical NF-kB-inhibited SCAPs. The odonto/osteogenic protein levels of SCAPs in control, inhibitor, MM, and inhibitor + MM groups were characterized by western blot analysis at day 7. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2014/319651/) licensed under a CC-BY license.

Western Blot: DMP-1 Antibody [NBP1-45525] - Analysis of DMP-1 in rat kidney tissue lysate.

Western Blot: DMP-1 Antibody [NBP1-45525] - Odonto/osteogenic differentiation in canonical NF-kB-activated SCAPs. Western blot analyses for the odonto/osteogenic proteins (BSP, OCN, RUNX2, OSX, DSP, OPN, DSP, and DMP-1) in different groups at day 3. beta-ACTIN served as an internal control. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2014/319651/) licensed under a CC-BY license.

Product Discontinued

DMP-1 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 21 of rat DMP-1 (EDL99499).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DMP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 0.5-4 ug/ml
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30755596).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 30% glycerol, 0.5% BSA
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for DMP-1 Antibody

• ARHP
• ARHR
• dentin matrix acidic phosphoprotein 1
• dentin matrix acidic phosphoprotein
• Dentin matrix protein 1
• DMP1
• DMP-1

Background

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DMP-1 Antibody (NBP1-45525)(10)

Publications using NBP1-45525

• Xu K, Liu Q, Huang W et al. Promotive Effect of FBXO32 on the Odontoblastic Differentiation of Human Dental Pulp Stem Cells International Journal of Molecular Sciences 2023-04-22 [PMID: 37175415] (WB, Human)
  
  Details:
  1:1000 dilution
• Liu M, Lingling C, Wu J, et al. Long Noncoding RNA MEG3 Expressed in Human Dental Pulp Regulates LPS-Induced Inflammation and Odontogenic Differentiation in Pulpitis Experimental cell research 2021-01-29 [PMID: 33524362] (WB, Human)
• Miyagawa K, Ohata Y, Delgado-Calle J et al. Osteoclast-derived IGF1 is required for pagetic lesion formation in vivo JCI Insight 2020-02-20 [PMID: 32078587] (Human)
• Li Z, Yan M, Yu Y et al. LncRNA H19 promotes the committed differentiation of stem cells from apical papilla via miR-141/SPAG9 pathway Cell Death Dis 2019-02-12 [PMID: 30755596] (ICC/IF, Human)
• Wang Y, Lu Y, Li Z et al. Oestrogen receptor a regulates the odonto/osteogenic differentiation of stem cells from apical papilla via ERK and JNK MAPK pathways Cell Prolif. 2018-08-02 [PMID: 30069950] (WB, Human)
• Wang Y, Zhou Y, Jin L et al. Mineral trioxide aggregate enhances the osteogenic capacity of periodontal ligament stem cells via NF-kB and MAPK signaling pathways J. Cell. Physiol. 2017-07-27 [PMID: 28749049] (WB, Human)
• Paudel U, Lee YH, Kwon TH et al. Eckols reduce dental pulp inflammation through the ERK1/2 pathway independent of COX-2 inhibition Oral Dis. 2014-06-13 [PMID: 24924779] (WB, Human)
  
  Details:
  Dentin matrix acidic phosphoprotein-1 /DMP1 antibody used for WB on lipopolysaccharide (LPS) stimulated human dental pulp cells/ HDPCs cultures treated or not with 10uM of 6-6 bieckol/EB1 or pholorofucofuroeckol-A /EB5 isolated from browin seaweed marine algae - Eisenia bicyclis (Figure 4 a)
• Li J, Yan M, Wang Z et al. Effects of Canonical NF- kappaB Signaling Pathway on the Proliferation and Odonto/Osteogenic Differentiation of Human Stem Cells from Apical Papilla. Biomed Res Int. 2014-05-27 [PMID: 24864235] (WB, Human)
• Huang H, Luo L, Li L et al. Calcium Phosphate Cement Promotes Odontoblastic Differentiation of Dental Pulp Cells In Vitro and In Vivo Coatings 2022-04-18 (IF/IHC, Rat)
• Jiang YL, Wang ZX, Liu XX et al. The Protective Effects of Osteocyte-Derived Extracellular Vesicles Against Alzheimer's Disease Diminished with Aging Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022-05-04 [PMID: 35508803] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: DMP1
• Entrez:
  • 1758(Human)
  • 13406(Mouse)
  • 25312(Rat)
• Uniprot:
  • D4A5C7(Rat)