DMAP1 Antibody

Images

 
Western Blot: DMAP1 Antibody [NBP2-03982] - Analysis of DMAP1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain using this antibody. 0 ug/ml, 1.0 ug/ml and ...read more
Immunohistochemistry-Paraffin: DMAP1 Antibody [NBP2-03982] - Analysis of DMAP1 in paraffin-embedded formalin-fixed mouse brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DMAP1 Antibody Summary

Immunogen
A portion of amino acids 250-300 of human DMAP1 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DMAP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0-4.0 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
The approximately 27 kD band seen in brain lysate is also seen in the prebleed and not thought to be relevant.

Reactivity Notes

Reacts with Chimpanzee, Cow, Dog, Human, Mouse and Rat. Predicted to react with Chicken and Zebrafish. The amino acid sequence used as immunogen is 100% homologous in Chimpanzee, Dog, Human, Rat, Cow, Mouse, 88% homologous in Chicken and 77% homologous in Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for DMAP1 Antibody

  • DNA methyltransferase 1 associated protein 1
  • DNA methyltransferase 1-associated protein 1
  • DNMAP1DNMT1-associated protein 1
  • DNMT1 associated protein 1
  • DNMTAP1
  • EAF2
  • FLJ11543
  • KIAA1425DKFZp686L09142
  • MEAF2
  • SWC4

Background

DNA methyltransferase 1-associated protein 1 (DMAP1) is involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The protein also specifically potentiates DAXX-mediated repression of glucocorticoid receptor-dependent transcription. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. DMAP1 is found in a wide variety of living organisms from yeasts to humans. The human DMAP1 gene is localized on chromosome 1p34.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DMAP1
Uniprot