DLL3 Antibody [Phycoerythrin]

U-118-MG human glioblastoma/astrocytoma cell line was stained with Goat Anti-Human DLL3 PE-conjugated Polyclonal Antibody (Catalog # FAB4315P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Phycoerythrin

DLL3 Antibody [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human DLL3
  Ala27-Pro488
  Accession # Q9NYJ7
• Specificity: Detects human DLL3 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) DLL1 is observed and less than 8% cross-reactivity with rhDLL4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DLL3
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLL3 Antibody [Phycoerythrin]

• delta (Drosophila)-like 3
• Delta3
• delta-like 3 (Drosophila)
• delta-like protein 3
• DLL3
• Drosophila Delta homolog 3
• Pudgy
• SCDO1
• SCDO1delta3

Background

DLL3 (Delta-like protein 3; also Delta3) is a 65‑67 kDa member of the Delta family of proteins. It is expressed in/on select cell types in the embryo, including somitic mesoderm, cochlear hair cells and noncycling cells of the neural tube that are undergoing differentiation. DLL3 is principally found in the Golgi apparatus and endosomes where it promotes Notch family receptor degradation. Mature human DLL3 is a 592 amino acid (aa) type I transmembrane protein. It possesses a 466 aa extracellular domain (aa 27-492) that contains a Notch-binding DSL domain (aa 176-215) followed by six EGF-like repeats (aa 216-465). There is one isoform variant that contains an Ala substitution for aa 588-618. Over aa 27-488, human DLL3 shares 84% aa identity with mouse DLL3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DLL3 Antibody (FAB4315P)(3)

Publications using FAB4315P

• Lin, S;Zhang, Y;Yao, J;Yang, J;Qiu, Y;Zhu, Z;Hua, H; DB-1314, a novel DLL3-targeting ADC with DNA topoisomerase I inhibitor, exhibits promising safety profile and therapeutic efficacy in preclinical small cell lung cancer models Journal of translational medicine 2024-08-14 [PMID: 39143619] (Flow Cytometry, Human)
• K Ohara, S Kinoshita, J Ando, Y Azusawa, M Ishii, S Harada, Y Mitsuishi, T Asao, K Tajima, T Yamamoto, F Takahashi, N Komatsu, K Takahashi, M Ando SCLC-J1, a novel small cell lung cancer cell line Biochemistry and Biophysics Reports, 2021-07-28;27(0):101089. 2021-07-28 [PMID: 34381882] (Flow Cytometry, Human)
• H Yu, W Hu, X Song, D Descalzi-M, Z Yang, R Korngold, Y Zhao Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria Int J Mol Sci, 2020-06-15;21(12):. 2020-06-15 [PMID: 32549211] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: DLL3
• Uniprot:
  • Q9NYJ7(Human)