DLK2/EGFL9 Antibody (908866R) [Unconjugated]

Recombinant Human DLK2/EGFL9 protein was serially diluted 2-fold and captured by Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB9756) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB97561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 908866R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DLK2/EGFL9 Antibody (908866R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell line HEK-293-derived transfected with human DLK2/EGFL9
  Asp27-Ser306
  Accession # Q6UY11
• Specificity: Detects human DLK2/EGFL9 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLK2/EGFL9 Antibody (908866R) [Unconjugated]

• delta-like 2 homolog (Drosophila)
• DLK2
• DLK-2
• EGFL9
• EGF-like protein 9
• EGF-like-domain, multiple 9
• Epidermal growth factor-like protein 9
• MGC111055
• MGC2487
• protein delta homolog 2

Background

DLK2 (protein delta homolog 2) also called Epidermal growth factor-like protein 9 (EGFL9) is a 383 aminoacid protein with a molecular weight of 40.5 kDa. DLK2 is a single-pass type I membrane protein and is a negative regulator in the Notch pathway. DLK2 regulates adipogenesis and 2 isoforms of the human protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q6UY11()