DGK-zeta Antibody [Unconjugated]

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human DGK‑ zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6099) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for DGK‑ zeta at approximately 124 & 114 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DGK-zeta Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human DGK‑ zeta
  Gln800-Val945
  Accession # Q13574
• Specificity: Detects human DGK‑ zeta in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DGKZ
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-zeta Antibody [Unconjugated]

• DAGK5
• DAGK6DAG kinase zeta
• DGKZ
• DGKzeta
• DGK-zeta
• diacylglycerol kinase zeta
• diacylglycerol kinase, zeta 104kDa
• diacylglycerol kinase, zeta
• Diglyceride kinase zeta
• EC 2.7.1.107
• hDGKzeta

Background

DGK-zeta (Diacylglycerol kinase zeta; also DAG kinase 6) is a 130 kDa member of the eukaryotic diacylglycerol (DAG) kinase family of proteins. It exists in both cytoplasm and nucleus, and is widely expressed, being found in neurons, skeletal muscle cells and endothelium. DGK-zeta converts DAG into phosphatidic acid (PA). In the nucleus, this activates mTOR and promotes cell proliferation. In the cytoplasm, DGK-zeta -generated PA activates PAK1, leading to increased cell motility. Human DGK-zeta is 1117 amino acids (aa) in length and contains two DAG-type Zn-finger regions (aa 287‑419), a MARCKS homology domain (aa 448‑462) and a catalytic domain (aa 486‑607). In addition to the 130 kDa protein, there are several splice variants that run about 114 kDa in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKZ
• Uniprot:
  • Q13574(Human)