Desmoglein-3 Antibody (DSG3/2838)

Images

 
Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2838) [NBP3-07876] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2838).
Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2838) [NBP3-07876] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2838).
Protein Array: Desmoglein-3 Antibody (DSG3/2838) [NBP3-07876] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2838).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P, PA
Clone
DSG3/2838
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Desmoglein-3 Antibody (DSG3/2838) Summary

Immunogen
Recombinant fragment (around aa 379-491) human Desmoglein-3 protein (exact sequence is proprietary) (Uniprot: P32926)
Localization
Cell Surface
Marker
Squamous Cell Marker
Specificity
Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This monoclonal antibody is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DSG3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08430)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Desmoglein-3 Antibody (DSG3/2838)

  • Cadherin family member 6
  • CDHF6
  • CDHF6130-kD pemphigus vulgaris antigen
  • desmoglein 3 (pemphigus vulgaris antigen)
  • desmoglein 3
  • Desmoglein3
  • Desmoglein-3
  • DKFZp686P23184,130 kDa pemphigus vulgaris antigen
  • DSG3
  • pemphigus vulgaris antigen
  • PVA

Background

Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DSG3
Uniprot