DEPTOR/DEPDC6 Antibody

Western Blot: DEPTOR/DEPDC6 Antibody [NBP2-27209] - Analysis of DEPTOR. MCF7 lysate in the (1) absence and (2) presence of immunizing peptide probed with 4 ug/ml of DEPTOR antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Product Discontinued

DEPTOR/DEPDC6 Antibody Summary

• Immunogen: A portion of amino acids 210-260 from human DEPTOR was used as the immunogen for this antibody.
• Predicted Species: Mouse (96%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DEPTOR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-5ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for DEPTOR/DEPDC6 Antibody

• DEP domain containing 6
• DEP domain containing mTOR interacting protein
• DEP domain containing MTOR-interacting protein
• DEP domain-containing mTOR-interacting protein
• DEP domain-containing protein 6
• DEP.6
• DEPDC6
• DEPDC6FLJ12341
• DEPTOR
• DKFZp564B1778
• FLJ12428
• FLJ13854

Background

DEPTOR, a component of the mTORC1 and mTORC2 complexes, is an inhibitor of mTOR. DEPTOR, along with mTOR, is one of five subunits of the rapamycin-sensitive mTORC1 complex or rapamycin-insensitive mTORC2 complex. mTOR itself is a PI3-kinase which plays a key role in maintenance of cellular homeostasis in response to microenvironments induced by growth factors, cell stress or nutrient conditions (1-3). While DEPTOR inhibits mTOR, in turn DEPTOR is repressed by mTORC1 and mTORC2. Thus control of mTOR, DEPTOR and related mTORC1 or mTORC2 pathways have become an important target for controlling cellular growth, or aberrant cellular growth in cancers, for example. In some multiple myelomas, unlike many cancers where DEPTOR expression is low, DEPTOR is overexpressed and PI3k and AkT high activities maintain cell survival (4). Interestingly the red wine polyphenol Resveratrol, a highly acclaimed treatment for cancer or cardiovascular disease management also appears to enhance mTOR and DEPTOR interaction, leading to mTOR inhibition (5). No surprise that many swear by the positive and beneficial effects of imbibing a glass of red wine with some frequency! Antibody to DEPTOR helps to clarify its expression and role in modulating mTOR driven growth of cancer cells for example.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DEPTOR
• Uniprot:
  • Q8TB45(Human)