DECR1 Antibody (OTI4G4) - Azide and BSA Free

Western Blot: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DECR1 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiDECR1.(1:2000)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Human lymphoma tissue using anti-DECR1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DECR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DECR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DECR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DECR1 mouse monoclonal antibody. (Heat induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using antiDECR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using antiDECR1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DECR1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DECR1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DECR1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: DECR1 Antibody (OTI4G4) - Azide and BSA Free [NBP2-70564] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-DECR1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI4G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

DECR1 Antibody (OTI4G4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DECR1 Antibody (OTI4G4) - Azide and BSA Free (NBP2-46449) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human DECR1 (NP_001350) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DECR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 32.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for DECR1 Antibody (OTI4G4) - Azide and BSA Free

• 2,4-dienoyl CoA reductase 1, mitochondrial
• 4-enoyl-CoA reductase [NADPH]
• 4-enoyl-CoA reductase
• DECR
• DECR1
• DECR2,4-dienoyl-CoA reductase [NADPH]2,4-dienoyl-CoA reductase, mitochondrial
• EC 1.3.1.34
• NADPH
• SDR18C1
• short chain dehydrogenase/reductase family 18C, member 1

Background

DECR1, also known as 2, 4-dienoyl-CoA reductase, mitochondrial, is a 36 kDa, 335 amino acid protein that catalyzes beta-oxidation in metabolism of unsaturated fatty enoyl-CoA esters with uneven double bonds. Current research is studying the involvement of the protein on diseases and disorders such as arthritis, malaria, dementia, nephropathy, brain disease, tuberculosis, anemia, and cancer. The protein interacts in metabolism, fatty acid biosynthesis, and beta oxidation pathways with other proteins such as HGS, PRKCE, ESR1, PTTG1, and SLC2A4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DECR1