DDX21 Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu, Ze, Rt, Po, Ca, Eq, Pm, RM, Sq
• Applications: WB, ChIP, Flow, ICC/IF, IHC, IP, ChIP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 647

DDX21 Antibody [Alexa Fluor™ Plus 647] Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 725 and the C-terminus (residue 783) of human DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21 using the numbering given in entry NP_004719.2 (GeneID 9188).
• Predicted Species: Rat (100%), Porcine (100%), Squirrel (100%), Rhesus Macaque (100%), Canine (100%), Equine (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, African elephant, Northern white-cheeked gibbon, and Thirteen-lined ground squirrel. Use in Zebrafish reported in scientific literature (PMID:32231306).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DDX21 Antibody [Alexa Fluor™ Plus 647]

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• DEAD box protein 21
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21
• DKFZp686F21172
• EC 3.6.1
• EC 3.6.4.13
• Gu protein
• GUA
• gu-alpha
• GURDB
• nucleolar RNA helicase 2
• Nucleolar RNA helicase Gu
• Nucleolar RNA helicase II
• RH II/Gu
• RH-II/GU
• RH-II/GuA
• RNA helicase II/Gu alpha

Background

DDX21 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. DDX21 has been shown to be required for the processing of 20S rRNA to 18S and has also been shown to be important for c-Jun transcriptional activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX21